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coolsnap es digital camera  (Nikon)


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    Nikon coolsnap es digital camera
    H pylori infection induces increased chemokine expression in gastric tissues and enhances the recruitment of DCs to the gastric epithelium. ( A ) Heatmap showing relative chemokine gene expression in human gastric antrum and body from noninfected (Co) and H pylori –infected donors without atrophy (gastritis, Ga) and with gastric atrophy (Atr) (n = 3 each). Data were extracted from Gene Expression Omnibus data set records GDS5338 (antrum) and GDS5411 (body). Arrow indicates significant up-regulation (analysis of variance with the Dunnett multiple comparisons test, P ≤ .05). ( B–D ) Gastric biopsy samples from H pylori –infected (n = 11) and noninfected (n = 8) human donors were immunolabeled for HLA-DR (Cy3, red) and epithelial cytokeratin (type 7/8; FITC, green). Cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Images were acquired on a Nikon Eclipse T2000-U fluorescent microscope equipped with a <t>CoolSnap</t> ES digital camera and NIS Elements BR2.30 software with a 20× objective. ( B ) Representative image of non– H pylori –infected mucosa. Arrows indicate examples of DCs in direct contact with epithelial cells. Scale bar : 20 μm. ( C ) The number of HLA-DR high DCs in direct contact with the basal side of the gastric epithelium was counted and normalized to epithelial length. Data from individual subjects, means ± SD, are shown. Data were analyzed by the Student t test. ( D ) The number of intraepithelial DCs was determined using the approach described for panel C .
    Coolsnap Es Digital Camera, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39694 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/coolsnap+es+digital+camera/NIS-Elements/pmc06599165-355-16-24
    Average 99 stars, based on 39694 article reviews
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    Images

    1) Product Images from "A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium"

    Article Title: A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    doi: 10.1016/j.jcmgh.2019.02.010

    H pylori infection induces increased chemokine expression in gastric tissues and enhances the recruitment of DCs to the gastric epithelium. ( A ) Heatmap showing relative chemokine gene expression in human gastric antrum and body from noninfected (Co) and H pylori –infected donors without atrophy (gastritis, Ga) and with gastric atrophy (Atr) (n = 3 each). Data were extracted from Gene Expression Omnibus data set records GDS5338 (antrum) and GDS5411 (body). Arrow indicates significant up-regulation (analysis of variance with the Dunnett multiple comparisons test, P ≤ .05). ( B–D ) Gastric biopsy samples from H pylori –infected (n = 11) and noninfected (n = 8) human donors were immunolabeled for HLA-DR (Cy3, red) and epithelial cytokeratin (type 7/8; FITC, green). Cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Images were acquired on a Nikon Eclipse T2000-U fluorescent microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software with a 20× objective. ( B ) Representative image of non– H pylori –infected mucosa. Arrows indicate examples of DCs in direct contact with epithelial cells. Scale bar : 20 μm. ( C ) The number of HLA-DR high DCs in direct contact with the basal side of the gastric epithelium was counted and normalized to epithelial length. Data from individual subjects, means ± SD, are shown. Data were analyzed by the Student t test. ( D ) The number of intraepithelial DCs was determined using the approach described for panel C .
    Figure Legend Snippet: H pylori infection induces increased chemokine expression in gastric tissues and enhances the recruitment of DCs to the gastric epithelium. ( A ) Heatmap showing relative chemokine gene expression in human gastric antrum and body from noninfected (Co) and H pylori –infected donors without atrophy (gastritis, Ga) and with gastric atrophy (Atr) (n = 3 each). Data were extracted from Gene Expression Omnibus data set records GDS5338 (antrum) and GDS5411 (body). Arrow indicates significant up-regulation (analysis of variance with the Dunnett multiple comparisons test, P ≤ .05). ( B–D ) Gastric biopsy samples from H pylori –infected (n = 11) and noninfected (n = 8) human donors were immunolabeled for HLA-DR (Cy3, red) and epithelial cytokeratin (type 7/8; FITC, green). Cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Images were acquired on a Nikon Eclipse T2000-U fluorescent microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software with a 20× objective. ( B ) Representative image of non– H pylori –infected mucosa. Arrows indicate examples of DCs in direct contact with epithelial cells. Scale bar : 20 μm. ( C ) The number of HLA-DR high DCs in direct contact with the basal side of the gastric epithelium was counted and normalized to epithelial length. Data from individual subjects, means ± SD, are shown. Data were analyzed by the Student t test. ( D ) The number of intraepithelial DCs was determined using the approach described for panel C .

    Techniques Used: Infection, Expressing, Immunolabeling, Labeling, Microscopy, Software

    Related Articles

    Staining:

    Article Title: MNPmApp: An image analysis tool to quantify mononuclear phagocyte distribution in mucosal tissues.
    Article Snippet: The slides were washed and cover-slipped with Fluoroshield histology mounting medium (Abcam) and sealed with nail varnish prior to microscopic analysis. .. Images of the stained TMA were acquired at 20 objective magnification on a Nikon Eclipse T2000-U microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software (Nikon, Tokyo, Japan) or on a Keyence BZ-X800 microscope (Keyence, Tukwila, WA). ..

    Microscopy:

    Article Title: MNPmApp: An image analysis tool to quantify mononuclear phagocyte distribution in mucosal tissues.
    Article Snippet: The slides were washed and cover-slipped with Fluoroshield histology mounting medium (Abcam) and sealed with nail varnish prior to microscopic analysis. .. Images of the stained TMA were acquired at 20 objective magnification on a Nikon Eclipse T2000-U microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software (Nikon, Tokyo, Japan) or on a Keyence BZ-X800 microscope (Keyence, Tukwila, WA). ..

    Article Title: MNPmApp: An image analysis tool to quantify mononuclear phagocyte distribution in mucosal tissues a, b
    Article Snippet: The slides were washed and cover-slipped with Fluoroshield histology mounting medium (Abcam) and sealed with nail varnish prior to microscopic analysis. .. Images were acquired at 20x objective magnification on a Nikon Eclipse T2000-U microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software (Nikon, Tokyo, Japan). ..

    Article Title: Photobleaching of melanosomes from retinal pigment epithelium: II. Effects on the response of living cells to photic stress.
    Article Snippet: Melanosomes of the retinal pigment epithelium (RPE) are long lived organelles that may undergo photobleaching with aging, which can diminish the antioxidant efficiency of melanin.. Here, isolated porcine RPE melanosomes were experimentally photobleached with visible light to simulate aging and compared with untreated granules or control particles (black latex beads) for their effects on the survival of photically stressed ARPE-19 cultures.. Particles were delivered to cultures for uptake by phagocytosis then cells were exposed to violet light and analyzed by a new live cell imaging method to identify the time of apoptotic blebbing as a dynamic measure of reduced cell survival.

    Article Title: A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium
    Article Snippet: .. Images were acquired on a Nikon Eclipse T2000-U fluorescent microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software with a 20× objective. ( B ) Representative image of non– H pylori –infected mucosa. ..

    Article Title: A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium.
    Article Snippet: Cryosections or a paraffin-embedded gastric tissue microarray7 (kind gift fromDr Paul Harris, Pontifical Catholic University of Chile, Santiago, Chile) prepared from human gastric tissue were labeled with anti–HLA-DR (cryosections: L243, BD 340689; tissue array: LN-3, ab166777; Abcam, Cambridge, UK) to stain DCs and with anticytokeratin FITC (cryosections: CAM5.2, BD 347653; tissue array: C11; 4545; Cell Signaling Technology, Danvers, MA) to stain epithelial cells, as previously described.4,7 Cell nuclei were stainedwith 40,6-diamidino-2-phenylindole. .. Samples were imaged on a Nikon Eclipse T2000-U fluorescent microscope (Nikon, Melville, NY) equippedwith a CoolSnap ES digital camera and NIS Elements BR2.30 (Nikon). .. ImageJ V1.4848 was used to measure epithelial cell length and count DCs that were in direct contact with the epithelium.

    Article Title: A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium
    Article Snippet: Cryosections or a paraffin-embedded gastric tissue microarray (kind gift from Dr Paul Harris, Pontifical Catholic University of Chile, Santiago, Chile) prepared from human gastric tissue were labeled with anti–HLA-DR (cryosections: L243, BD 340689; tissue array: LN-3, ab166777; Abcam, Cambridge, UK) to stain DCs and with anticytokeratin FITC (cryosections: CAM5.2, BD 347653; tissue array: C11; 4545; Cell Signaling Technology, Danvers, MA) to stain epithelial cells, as previously described., Cell nuclei were stained with 4′,6-diamidino-2-phenylindole. .. Samples were imaged on a Nikon Eclipse T2000-U fluorescent microscope (Nikon, Melville, NY) equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 (Nikon). .. ImageJ V1.48 was used to measure epithelial cell length and count DCs that were in direct contact with the epithelium.

    Software:

    Article Title: MNPmApp: An image analysis tool to quantify mononuclear phagocyte distribution in mucosal tissues.
    Article Snippet: The slides were washed and cover-slipped with Fluoroshield histology mounting medium (Abcam) and sealed with nail varnish prior to microscopic analysis. .. Images of the stained TMA were acquired at 20 objective magnification on a Nikon Eclipse T2000-U microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software (Nikon, Tokyo, Japan) or on a Keyence BZ-X800 microscope (Keyence, Tukwila, WA). ..

    Article Title: MNPmApp: An image analysis tool to quantify mononuclear phagocyte distribution in mucosal tissues a, b
    Article Snippet: The slides were washed and cover-slipped with Fluoroshield histology mounting medium (Abcam) and sealed with nail varnish prior to microscopic analysis. .. Images were acquired at 20x objective magnification on a Nikon Eclipse T2000-U microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software (Nikon, Tokyo, Japan). ..

    Article Title: A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium
    Article Snippet: .. Images were acquired on a Nikon Eclipse T2000-U fluorescent microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software with a 20× objective. ( B ) Representative image of non– H pylori –infected mucosa. ..

    Infection:

    Article Title: A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium
    Article Snippet: .. Images were acquired on a Nikon Eclipse T2000-U fluorescent microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software with a 20× objective. ( B ) Representative image of non– H pylori –infected mucosa. ..



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    H pylori infection induces increased chemokine expression in gastric tissues and enhances the recruitment of DCs to the gastric epithelium. ( A ) Heatmap showing relative chemokine gene expression in human gastric antrum and body from noninfected (Co) and H pylori –infected donors without atrophy (gastritis, Ga) and with gastric atrophy (Atr) (n = 3 each). Data were extracted from Gene Expression Omnibus data set records GDS5338 (antrum) and GDS5411 (body). Arrow indicates significant up-regulation (analysis of variance with the Dunnett multiple comparisons test, P ≤ .05). ( B–D ) Gastric biopsy samples from H pylori –infected (n = 11) and noninfected (n = 8) human donors were immunolabeled for HLA-DR (Cy3, red) and epithelial cytokeratin (type 7/8; FITC, green). Cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Images were acquired on a Nikon Eclipse T2000-U fluorescent microscope equipped with a <t>CoolSnap</t> ES digital camera and NIS Elements BR2.30 software with a 20× objective. ( B ) Representative image of non– H pylori –infected mucosa. Arrows indicate examples of DCs in direct contact with epithelial cells. Scale bar : 20 μm. ( C ) The number of HLA-DR high DCs in direct contact with the basal side of the gastric epithelium was counted and normalized to epithelial length. Data from individual subjects, means ± SD, are shown. Data were analyzed by the Student t test. ( D ) The number of intraepithelial DCs was determined using the approach described for panel C .
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    Image Search Results


    H pylori infection induces increased chemokine expression in gastric tissues and enhances the recruitment of DCs to the gastric epithelium. ( A ) Heatmap showing relative chemokine gene expression in human gastric antrum and body from noninfected (Co) and H pylori –infected donors without atrophy (gastritis, Ga) and with gastric atrophy (Atr) (n = 3 each). Data were extracted from Gene Expression Omnibus data set records GDS5338 (antrum) and GDS5411 (body). Arrow indicates significant up-regulation (analysis of variance with the Dunnett multiple comparisons test, P ≤ .05). ( B–D ) Gastric biopsy samples from H pylori –infected (n = 11) and noninfected (n = 8) human donors were immunolabeled for HLA-DR (Cy3, red) and epithelial cytokeratin (type 7/8; FITC, green). Cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Images were acquired on a Nikon Eclipse T2000-U fluorescent microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software with a 20× objective. ( B ) Representative image of non– H pylori –infected mucosa. Arrows indicate examples of DCs in direct contact with epithelial cells. Scale bar : 20 μm. ( C ) The number of HLA-DR high DCs in direct contact with the basal side of the gastric epithelium was counted and normalized to epithelial length. Data from individual subjects, means ± SD, are shown. Data were analyzed by the Student t test. ( D ) The number of intraepithelial DCs was determined using the approach described for panel C .

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium

    doi: 10.1016/j.jcmgh.2019.02.010

    Figure Lengend Snippet: H pylori infection induces increased chemokine expression in gastric tissues and enhances the recruitment of DCs to the gastric epithelium. ( A ) Heatmap showing relative chemokine gene expression in human gastric antrum and body from noninfected (Co) and H pylori –infected donors without atrophy (gastritis, Ga) and with gastric atrophy (Atr) (n = 3 each). Data were extracted from Gene Expression Omnibus data set records GDS5338 (antrum) and GDS5411 (body). Arrow indicates significant up-regulation (analysis of variance with the Dunnett multiple comparisons test, P ≤ .05). ( B–D ) Gastric biopsy samples from H pylori –infected (n = 11) and noninfected (n = 8) human donors were immunolabeled for HLA-DR (Cy3, red) and epithelial cytokeratin (type 7/8; FITC, green). Cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Images were acquired on a Nikon Eclipse T2000-U fluorescent microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software with a 20× objective. ( B ) Representative image of non– H pylori –infected mucosa. Arrows indicate examples of DCs in direct contact with epithelial cells. Scale bar : 20 μm. ( C ) The number of HLA-DR high DCs in direct contact with the basal side of the gastric epithelium was counted and normalized to epithelial length. Data from individual subjects, means ± SD, are shown. Data were analyzed by the Student t test. ( D ) The number of intraepithelial DCs was determined using the approach described for panel C .

    Article Snippet: Samples were imaged on a Nikon Eclipse T2000-U fluorescent microscope (Nikon, Melville, NY) equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 (Nikon).

    Techniques: Infection, Expressing, Immunolabeling, Labeling, Microscopy, Software